STUDIES ON L-VALINE FERMENTATION PART I. PRODUCTION OF L-VALINE BY AEROBACTER BACTERIA

Vibrational spectroscopic studies of L,D-alternating valine peptides

Raman and infrared spectra of Boc-(L-Val-D-Val),-OMe [(LD-Val),], with n = 4, 6 and 8, were obtained in crystalline and solution states. Based on predictions from normal-mode calculations of a number of relevant singleand double-stranded p-helix conformations, structures were assigned for the three oligopeptides under the abovementioned conditions. In the crystalline state, all three oligopepti...

L-valine production with pyruvate dehydrogenase complex-deficient Corynebacterium glutamicum.

Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE sh...

A non-planar peptide bond in L-seryl-L-valine.

The molecular structure of (I) is shown in Fig. 1. Bond lengths and angles are normal, but the unusual non-planarity of the peptide bond is quite evident. The associated torsion angle C1ÐC3ÐN2ÐC4 (!) is 157.37 (15) , a deviation from 180 that is superceded among small chiral peptides only by the 156.6 ! angle in N-(tert-butoxycarbonyl)-l-Pro-l-Leu benzyl ester (Sugino et al., 1978). Fig. 2(a) s...

Uptake of L-valyl-L-valine and glycylsarcosine by hamster jejunum in vitro.

Metabolic function of Corynebacterium glutamicum aminotransferases AlaT and AvtA and impact on L-valine production.

Aminotransferases (ATs) interacting with L-alanine are the least studied bacterial ATs. Whereas AlaT converts pyruvate to L-alanine in a glutamate-dependent reaction, AvtA is able to convert pyruvate to L-alanine in an L-valine-dependent manner. We show here that the wild type of Corynebacterium glutamicum with a deletion of either of the corresponding genes does not exhibit an explicit growth ...